RNA Interference and Small RNA Analysis | Zendy

Chengjian Li | Zendy; Phillip D. Zamore | Zendy

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RNA Interference and Small RNA Analysis

Author(s) -

Chengjian Li,

Phillip D. Zamore

Publication year - 2019

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.top097436

Subject(s) - rna interference , rna silencing , microrna , rna , small rna , trans acting sirna , dna directed rna interference , computational biology , gene silencing , function (biology) , rna induced silencing complex , biology , small interfering rna , gene expression , genetics , gene

Small silencing RNAs have provided powerful reverse genetics tools and have opened new areas of research. This introduction describes the use of RNAi to suppress expression of individual genes for loss-of-function analysis. It also summarizes methods for measuring specific and global changes in small RNA expression, as well as methods to inhibit the function of individual endogenous small RNA species such as miRNAs.

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