Elementary Protein Analysis in Schizosaccharomyces pombe | Zendy

Ágnes Grallert | Zendy; Iain Hagan | Zendy

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Elementary Protein Analysis in Schizosaccharomyces pombe

Author(s) -

Ágnes Grallert,

Iain Hagan

Publication year - 2017

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.top079806

Subject(s) - schizosaccharomyces pombe , proteome , yeast , computational biology , schizosaccharomyces , model organism , fission , biology , function (biology) , organism , saccharomyces cerevisiae , microbiology and biotechnology , genetics , physics , gene , quantum mechanics , neutron

Biochemical monitoring and interrogation of protein function is a critical component of most fission yeast studies. In particular, its small proteome size, high conservation of core molecular cell biology, and genetic malleability make Schizosaccharomyces pombe an excellent model organism in which to use mass spectrometry to conduct proteome-wide approaches. Here we discuss issues encountered during the analysis of fission yeast protein preparations.

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