Imaging Intracellular Signaling Using Two-Photon Fluorescent Lifetime Imaging Microscopy | Zendy

Ryohei Yasuda | Zendy

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Imaging Intracellular Signaling Using Two-Photon Fluorescent Lifetime Imaging Microscopy

Author(s) -

Ryohei Yasuda

Publication year - 2012

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.top072090

Subject(s) - förster resonance energy transfer , two photon excitation microscopy , microscopy , intracellular , fluorescence lifetime imaging microscopy , fluorescence , live cell imaging , fluorescence microscope , confocal microscopy , fluorescence in the life sciences , materials science , biophysics , chemistry , microbiology and biotechnology , optics , biology , physics , cell , biochemistry

The recent development of Förster resonance energy transfer (FRET) sensors and FRET imaging techniques permits visualization of the dynamics of intracellular signaling events with high spatiotemporal resolution. In particular, fluorescence lifetime imaging in combination with two-photon laser-scanning microscopy (two-photon fluorescence lifetime imaging microscopy [2pFLIM]) is a powerful tool to monitor signaling events in small subcellular compartments in thick tissue. This article provides practical guidelines for quantitative imaging of intracellular signaling using 2pFLIM.

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