Open AccessFluorescence Microscopy
Author(s) -
Michael J. Sanderson,
Ian F. Smith,
Ian Parker,
Martin D. Bootman
Publication year - 2014
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.top071795
Subject(s) - microscopy , light sheet fluorescence microscopy , fluorescence microscope , microscope , total internal reflection fluorescence microscope , confocal microscopy , super resolution microscopy , scanning confocal electron microscopy , optical microscope , resolution (logic) , photoactivated localization microscopy , optics , two photon excitation microscopy , optical sectioning , laser microscopy , materials science , confocal , fluorescence , computer science , physics , scanning electron microscope , artificial intelligence
Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution. The basics of wide-field microscopy are outlined to emphasize the selection, advantages, and correct use of laser scanning confocal microscopy, two-photon microscopy, scanning disk confocal microscopy, total internal reflection, and super-resolution microscopy. In addition, the principles of how these microscopes form images are reviewed to appreciate their capabilities, limitations, and constraints for operation.
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