In Vivo Imaging of Synaptogenesis in Zebrafish | Zendy

James D. Jontes | Zendy; Michelle R. Emond | Zendy

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In Vivo Imaging of Synaptogenesis in Zebrafish

Author(s) -

James D. Jontes,

Michelle R. Emond

Publication year - 2012

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.top069237

Subject(s) - zebrafish , synaptogenesis , green fluorescent protein , embryonic stem cell , nervous system , preclinical imaging , neuroscience , model system , embryogenesis , biology , embryo , microbiology and biotechnology , vertebrate , in vivo , fluorescent protein , computational biology , gene , genetics

The embryonic zebrafish is a nearly ideal model system in which to use time-lapse imaging to study the development of the vertebrate nervous system in vivo. The embryos are small and transparent, they develop externally and rapidly, and the embryonic central nervous system is relatively simple and highly stereotyped. With the refinement of green fluorescent protein (GFP) as a genetically encoded fluorescent tag of neuronal proteins, along with advances in imaging technology, it is possible to follow the cellular and molecular events underlying development as they occur in the living embryo. This article describes strategies for imaging synapse formation in the embryonic zebrafish.

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