Patch-Clamp Recording of Voltage-Sensitive Ca2+ Channels | Zendy

María A. Gandini | Zendy; Alejandro Sandoval | Zendy; Ricardo Felix | Zendy

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Patch-Clamp Recording of Voltage-Sensitive Ca²⁺ Channels

Author(s) -

María A. Gandini,

Alejandro Sandoval,

Ricardo Felix

Publication year - 2014

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.top066092

Subject(s) - patch clamp , voltage clamp , ion channel , clamp , current clamp , voltage , ionic bonding , biophysics , current (fluid) , chemistry , electrophysiology , computer science , membrane potential , nanotechnology , materials science , ion , neuroscience , biology , electrical engineering , biochemistry , engineering , computer graphics (images) , receptor , clamping , organic chemistry

In this article, we focus on a refinement of the traditional voltage-clamp methods that are used to measure current from whole cells, or relatively large areas of membrane, called the patch-clamp technique. Although this technique has extended the application of voltage-clamp methods to the recording of ionic currents flowing through single channels, in its whole-cell configuration it has become the most widely used method for recording ionic currents. We give particular attention to the study of voltage-gated (Ca V ) Ca 2+ channels using the patch-clamp technique and discuss some aspects of the molecular physiology of these proteins.

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