Labeling Nuclear DNA with Hoechst 33342 | Zendy

Brad Chazotte | Zendy

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Labeling Nuclear DNA with Hoechst 33342

Author(s) -

Brad Chazotte

Publication year - 2011

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot5557

Subject(s) - interphase , dna , nuclear dna , minor groove , fluorescence , chemistry , stain , mitosis , microbiology and biotechnology , fluorescence microscope , thymine , biophysics , staining , biology , biochemistry , genetics , gene , physics , quantum mechanics , mitochondrial dna

A number of fluorescent stains are available that label DNA and allow easy visualization of the nucleus in interphase cells and chromosomes in mitotic cells. One advantage of Hoechst 33342 is that it is membrane permeant and, thus, can stain live cells. Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture.

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