Imaging Cell Movements in Egg-Cylinder Stage Mouse Embryos | Zendy

Shankar Srinivas | Zendy

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Imaging Cell Movements in Egg-Cylinder Stage Mouse Embryos

Author(s) -

Shankar Srinivas

Publication year - 2010

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot5539

Subject(s) - epiblast , primitive streak , endoderm , embryo , biology , anatomy , gastrulation , microbiology and biotechnology , embryogenesis , stage (stratigraphy) , embryonic stem cell , genetics , paleontology , gene

Cell movements in the pregastrulation egg cylinder mouse embryo play an important role in patterning. The stereotypic movement of the anterior visceral endoderm converts a proximal-distal axis to an anteroposterior axis by properly positioning the primitive streak. The epiblast at this stage is also characterized by a great deal of cell mixing, about which very little is known. Visualizing such cell movements can help us understand their role in embryonic development. This protocol describes a method to isolate and culture the egg cylinder-stage mouse embryo, as well as an approach for time-lapse imaging of embryos cultured in vivo.

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