Chemical and UV Mutagenesis of Spores and Protonemal Tissue from the Moss Physcomitrella patens | Zendy

David J. Cove | Zendy; PierreFrançois Perroud | Zendy; Audra J. Charron | Zendy; Stuart F. McDaniel | Zendy; Abha Khandelwal | Zendy; Ralph S. Quatrano | Zendy

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Chemical and UV Mutagenesis of Spores and Protonemal Tissue from the Moss Physcomitrella patens

Author(s) -

David J. Cove,

PierreFrançois Perroud,

Audra J. Charron,

Stuart F. McDaniel,

Abha Khandelwal,

Ralph S. Quatrano

Publication year - 2009

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot5142

Subject(s) - physcomitrella patens , mutagenesis , spore , mutagen , ethyl methanesulfonate , moss , chemistry , ultraviolet light , biology , botany , mutant , biochemistry , gene , carcinogen , photochemistry

This protocol describes how to mutagenize spores and protonemal tissue from moss (Physcomitrella patens) using chemicals or ultraviolet (UV) light. Spores are mutagenized using the alkylating agents N-methyl-N′-nitro-N-nitrosoguanidine (NTG) and ethyl methanesulfonate (EMS), and protonemal tissue is mutagenized with NTG and UV light. Compared to alkylating agents, UV is less effective as a mutagen, but it may be advantageous because it is less hazardous and may not lead to clustered lesions.

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