Culturing Human Embryonic Stem Cells in Feeder-Free Conditions: Figure 1. | Zendy

Sohyun L. McElroy | Zendy; Renee A. Reijo Pera | Zendy

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Culturing Human Embryonic Stem Cells in Feeder-Free Conditions: Figure 1.

Author(s) -

Sohyun L. McElroy,

Renee A. Reijo Pera

Publication year - 2008

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot5044

Subject(s) - embryonic stem cell , microbiology and biotechnology , matrigel , germ layer , kosr , cell culture , stem cell , biology , in vitro , chemistry , adult stem cell , induced pluripotent stem cell , genetics , gene

Human embryonic stem cells (hESCs) have the potential to differentiate into all three germ layers and proliferate in long-term culture in vitro. hESCs can provide a cell source for the testing of novel therapies, drug screening, and functional genomics applications. Undifferentiated hESCs can be maintained and proliferated on mouse embryonic fibroblasts (MEFs) or human feeder cells. In this protocol, we describe the culture of hESCs in feeder-free conditions on Matrigel with MEF-conditioned medium. This protocol can be used for applications such as genetic modification of hESCs without feeder cell contamination.

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