Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi): Figure 1. | Zendy

Leonie Misquitta | Zendy; Wei Qin | Zendy; Bruce M. Paterson | Zendy

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Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi): Figure 1.

Author(s) -

Leonie Misquitta,

Wei Qin,

Bruce M. Paterson

Publication year - 2008

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot4918

Subject(s) - rna interference , embryo , rna silencing , drosophila (subgenus) , microbiology and biotechnology , biology , gene , interference (communication) , drosophila melanogaster , gene expression , genetics , rna , computer science , computer network , channel (broadcasting)

RNA interference (RNAi) is a powerful method for determining the role of specific genes during Drosophila embryogenesis. This protocol describes a technique by which Drosophila embryos can be injected with dsRNA in order to disrupt targeted gene function. The approach is straightforward, utilizing improved methods for injecting the dsRNA directly through the chorion of the embryo. This strategy minimizes problems normally associated with desiccation of the dechorionated embryo and facilitates post-injection analysis of gene expression.

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