Immunohistochemistry of Whole-Mount Mouse Embryos | Zendy

Alex Joyner | Zendy; Nancy A. Wall | Zendy

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Immunohistochemistry of Whole-Mount Mouse Embryos

Author(s) -

Alex Joyner,

Nancy A. Wall

Publication year - 2008

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot4820

Subject(s) - embryo , immunohistochemistry , antibody , biology , antigen , specific antibody , microbiology and biotechnology , anatomy , andrology , immunology , medicine

The technique presented this protocol is ideal for obtaining an overall, three-dimensional picture of the distribution of an antigen in embryos from the prestreak stage to ~10.5 dpc (days post-coitum). The embryos are fixed and permeabilized so that antibodies can penetrate all of the tissues. However, antibodies will not penetrate the embryo completely if it is too large, and so it is better to dissect specific organs (e.g., brain, lungs, and gut) and process them separately.

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