Open AccessMapping Protein Distributions on Polytene Chromosomes by Immunostaining
Author(s) -
Renato Paro
Publication year - 2008
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.prot4714
Subject(s) - polytene chromosome , immunostaining , biology , giemsa stain , genetics , drosophila melanogaster , microbiology and biotechnology , evolutionary biology , computational biology , gene , immunohistochemistry , immunology
The formidable size and structure of polytene chromosomes allow mapping of chromosomal protein distributions at very high resolution. This protocol describes the preparation of polytene chromosomes from Drosophila larvae, immunostaining of the chromosomes with a protein of interest, and counterstaining with Giemsa and Hoechst.
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