Raising Antibodies for Use in Xenopus | Zendy

Maya Z. Piccinni | Zendy; Matthew Guille | Zendy

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Raising Antibodies for Use in Xenopus

Author(s) -

Maya Z. Piccinni,

Matthew Guille

Publication year - 2020

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot105585

Subject(s) - immunogen , xenopus , antibody , zebrafish , hek 293 cells , raising (metalworking) , embryonic stem cell , microbiology and biotechnology , chemistry , computational biology , biology , immunology , monoclonal antibody , gene , genetics , materials science , metallurgy

For work in Xenopus , frog-specific antibodies must usually be raised, although a few antibodies against mammalian proteins cross-react. To produce an immunogen for antibody production, human embryonic kidney (HEK) expression systems can be used as described here. For most laboratories, the actual method of raising the antibody is determined by local ethical regulations controlling the adjuvant and injection protocols used. Because these steps are often outsourced, they are not included in this protocol.

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