Live Imaging of Tardigrade Embryonic Development by Differential Interference Contrast Microscopy | Zendy

Kira L. Heikes | Zendy; Bob Goldstein | Zendy

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Live Imaging of Tardigrade Embryonic Development by Differential Interference Contrast Microscopy

Author(s) -

Kira L. Heikes,

Bob Goldstein

Publication year - 2018

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot102335

Subject(s) - differential interference contrast microscopy , tardigrade , microscopy , embryonic stem cell , microbiology and biotechnology , biology , contrast (vision) , phase contrast microscopy , embryogenesis , anatomy , embryo , optics , physics , zoology , genetics , gene

The tardigrade Hypsibius exemplaris was chosen as a model system in part because embryos and animals are optically clear at all stages, facilitating the viewing and filming of internal processes. Multiplane video recordings under differential interference contrast (DIC) microscopy have allowed early embryonic cell lineages to be reconstructed through seven rounds of division and have revealed invariant patterns of asymmetric cell divisions, nuclear migrations, and cell migrations. Here, we present a protocol for filming embryonic development of H. exemplaris by DIC microscopy.

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