Purification of Total RNA from Mammalian Cells and Tissues | Zendy

Michael R. Green | Zendy; Joseph Sambrook | Zendy

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Purification of Total RNA from Mammalian Cells and Tissues

Author(s) -

Michael R. Green,

Joseph Sambrook

Publication year - 2020

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot101659

Subject(s) - lysis , rna , reagent , chemistry , suspension (topology) , isolation (microbiology) , biochemistry , glycogen , microbiology and biotechnology , monolayer , cell , chromatography , biology , gene , organic chemistry , mathematics , homotopy , pure mathematics

This protocol describes the use of a monophasic lysis reagent to isolate total RNA from mammalian cells (grown as either a monolayer or in suspension) or tissues. An alternative protocol for the isolation of RNA from a small quantity of cells (10 2 –10 4 ) or tissue (1–10 mg) is also included. In the latter case, glycogen is added to the monophasic lysis reagent and is used as a carrier to increase the recovery of RNA.

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