Cloning Polymerase Chain Reaction (PCR) Products: TOPO TA Cloning | Zendy

Michael R. Green | Zendy; Joseph Sambrook | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Cloning Polymerase Chain Reaction (PCR) Products: TOPO TA Cloning

Author(s) -

Michael R. Green,

Joseph Sambrook

Publication year - 2021

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot101311

Subject(s) - cloning (programming) , polymerase chain reaction , computational biology , molecular cloning , cloning vector , biology , microbiology and biotechnology , computer science , genetics , gene , complementary dna , programming language

This protocol describes the use of TOPO-activated TA vectors for cloning. Manufacturers of cloning kits provide excellent manuals that explain in detail what to do and why to do it. This makes TOPO cloning easy, but not foolproof. When setting up TOPO cloning for the first time, set up a trial experiment as described here.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research