Open AccessSouthern Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Membranes
Author(s) -
Michael R. Green,
Joseph Sambrook
Publication year - 2021
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.prot100495
Subject(s) - membrane , nucleic acid , chemistry , southern blot , molecular probe , blot , hybridization probe , microbiology and biotechnology , stripping (fiber) , biochemistry , chromatography , dna , biology , materials science , gene , composite material
In this protocol, restriction fragments that have been transferred to a membrane by Southern blotting are hybridized to a labeled probe. Methods for stripping the probe from the membrane are also included.
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