Repetitive Immunization at Multiple Sites (RIMMS) of Mice, Rats, and Hamsters | Zendy

Edward Greenfield | Zendy

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Repetitive Immunization at Multiple Sites (RIMMS) of Mice, Rats, and Hamsters

Author(s) -

Edward Greenfield

Publication year - 2020

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot100313

Subject(s) - immunization , antigen , epitope , antibody , immunology , biology , immune system , innate immune system , repertoire , animal model , virology , physics , acoustics , endocrinology

The repetitive immunization at multiple sites (RIMMS) protocol capitalizes on the animal's innate immune system, which is genetically preprogrammed to recognize many antigens. By repetitively immunizing the animal, B cells that recognize the antigen are kept continuously expanding until the lymph nodes are harvested for hybridoma generation. This is a good method for making a more diverse repertoire of antibodies or antibodies directed against conformational epitopes.

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