Purification of Labeled Antibodies Using Size-Exclusion Chromatography | Zendy

Eric A. Berg | Zendy; Jordan B. Fishman | Zendy

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Purification of Labeled Antibodies Using Size-Exclusion Chromatography

Author(s) -

Eric A. Berg,

Jordan B. Fishman

Publication year - 2020

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot099358

Subject(s) - size exclusion chromatography , chromatography , conjugate , ionic strength , chemistry , ion chromatography , ion exchange , antibody , ion , biochemistry , mathematics , biology , organic chemistry , enzyme , immunology , mathematical analysis , aqueous solution

Many antibody labeling procedures call for a desalting or purification step requiring size-exclusion chromatography (SEC). The method outlined here contains information needed to desalt an antibody conjugate. Similar procedures would be used for ion-exchange chromatography using a gradient of increasing ionic strength. Resins can be purchased in bulk (as in this protocol), or commercially available columns are available.

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