Conjugation of Antibodies to Horseradish Peroxidase | Zendy

Eric A. Berg | Zendy; Jordan B. Fishman | Zendy

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Conjugation of Antibodies to Horseradish Peroxidase

Author(s) -

Eric A. Berg,

Jordan B. Fishman

Publication year - 2019

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot099309

Subject(s) - horseradish peroxidase , conjugated system , chemistry , peroxidase , antibody , combinatorial chemistry , biochemistry , enzyme , biophysics , organic chemistry , biology , immunology , polymer

Antibodies conjugated with horseradish peroxidase (HRP) are one of the most widely used bioreagents in the biological sciences. This protocol is a basic method for adding HRP to a thiolated antibody and can be adapted for use with different cross-linkers. Conjugation methods usually focus on linking through the lysines on HRP because there are only six of them and their modification does not adversely affect enzyme activity.

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