Immunoprecipitation | Zendy

James A. DeCaprio | Zendy; Thomas O. Kohl | Zendy

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Immunoprecipitation

Author(s) -

James A. DeCaprio,

Thomas O. Kohl

Publication year - 2017

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot098640

Subject(s) - immunoprecipitation , lysis , blot , chemistry , microbiology and biotechnology , antibody , specific antibody , mass spectrometry , antigen , chromatography , computational biology , biochemistry , biology , gene , immunology

This immunoprecipitation protocol details individual steps for the enrichment and purification process of specific proteins from a complex cell lysate using an antibody bound to a solid matrix. Purified antigen(s) can be eluted by various methods, and the resultant protein target can be analyzed and/or identified by numerous assays, including the enzyme-linked immunosorbent assay (ELISA), western blotting, or mass spectrometry.

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