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Adoptive cell transfer from inbred adult  Xenopus  to inbred tadpoles is a useful way to study the dissemination of immune cells or pathogen-infected immune cells in tadpoles. For example,  Xenopus  peritoneal leukocytes (PLs) can be readily infected by pathogens such as Frog virus 3 (FV3) and  Mycobacterium marinum  ( M. marinum ). By transferring fluorescently labeled, FV3-infected PLs into tadpoles, we observed infiltration of these cells into the tadpole's brain, which indicates that FV3-infected PLs can cross blood brain barrier. Taking advantage of tadpoles’ transparency, fluorescently labeled immune cells can be tracked in real time using fluorescence microscopy.

Adoptive Transfer of Fluorescently Labeled Immune Cells in Xenopus