Adoptive Transfer of Fluorescently Labeled Immune Cells in Xenopus | Zendy

Kun Hyoe Rhoo | Zendy; Jacques Robert | Zendy

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Adoptive Transfer of Fluorescently Labeled Immune Cells in Xenopus

Author(s) -

Kun Hyoe Rhoo,

Jacques Robert

Publication year - 2018

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot097592

Subject(s) - xenopus , immune system , biology , adoptive cell transfer , pathogen , tadpole (physics) , microbiology and biotechnology , immunology , t cell , genetics , physics , particle physics , gene

Adoptive cell transfer from inbred adult Xenopus to inbred tadpoles is a useful way to study the dissemination of immune cells or pathogen-infected immune cells in tadpoles. For example, Xenopus peritoneal leukocytes (PLs) can be readily infected by pathogens such as Frog virus 3 (FV3) and Mycobacterium marinum ( M. marinum ). By transferring fluorescently labeled, FV3-infected PLs into tadpoles, we observed infiltration of these cells into the tadpole's brain, which indicates that FV3-infected PLs can cross blood brain barrier. Taking advantage of tadpoles’ transparency, fluorescently labeled immune cells can be tracked in real time using fluorescence microscopy.

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