Cranial Neural Crest Explants | Zendy

Hélène Cousin | Zendy; Dominique Alfandari | Zendy

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Cranial Neural Crest Explants

Author(s) -

Hélène Cousin,

Dominique Alfandari

Publication year - 2018

Publication title -

cold spring harbor protocols

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot097394

Subject(s) - explant culture , neural crest , cranial neural crest , xenopus , in vitro , crest , anatomy , biology , computer science , microbiology and biotechnology , embryo , genetics , physics , gene , quantum mechanics

The cranial neural crest (CNC) explant assay was originally designed to assess the basic requirements for CNC migration in vitro. This protocol describes the key parameters of CNC explants in Xenopus laevis , with a focus on how to extirpate CNC cells and assay their migration in vitro. The protocol can be adapted according to the needs of the experimenter, some examples of which are discussed here.

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