Isolation of Xenopus Oocytes | Zendy

Karen Newman | Zendy; Tristan Agüero | Zendy; Mary Lou King | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Isolation of Xenopus Oocytes

Author(s) -

Karen Newman,

Tristan Agüero,

Mary Lou King

Publication year - 2018

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot095851

Subject(s) - xenopus , oocyte , isolation (microbiology) , ovary , andrology , biology , microbiology and biotechnology , chemistry , bioinformatics , endocrinology , medicine , genetics , embryo , gene

Xenopus oocytes and oocyte extracts are the starting material for a variety of experimental approaches. Oocytes are obtained by surgical removal of the ovary from anesthetized females. Although oocytes may be used while they remain within their ovarian follicle, it is more practical to work with defolliculated oocytes. Defolliculation can be performed either manually or enzymatically. Here we present a protocol for the isolation and separation of Xenopus oocytes at various developmental stages, and guidelines for maintaining oocytes in culture.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research