Open AccessThe Basic Polymerase Chain Reaction (PCR)
Author(s) -
Michael R. Green,
Joseph Sambrook
Publication year - 2018
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.prot095117
Subject(s) - polymerase chain reaction optimization , polymerase chain reaction , inverse polymerase chain reaction , hot start pcr , microbiology and biotechnology , polymerase , chemistry , dna , multiplex polymerase chain reaction , real time polymerase chain reaction , dna polymerase , biology , biochemistry , gene
This protocol describes the reagents and procedures required to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase. It is the foundation for all subsequent variations of the polymerase chain reaction (PCR).
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom