Whole-Genome Sequencing: Manual Library Preparation | Zendy

Elaine R. Mardis | Zendy; W. Richard McCombie | Zendy

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Whole-Genome Sequencing: Manual Library Preparation

Author(s) -

Elaine R. Mardis,

W. Richard McCombie

Publication year - 2016

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot094615

Subject(s) - genomic library , genomic dna , polymerase chain reaction , illumina dye sequencing , dna sequencing , multiple displacement amplification , sequencing by ligation , dna , computational biology , genome , biology , microbiology and biotechnology , genetics , dna extraction , gene , base sequence

This protocol describes a manual approach for the preparation of genomic DNA libraries suitable for Illumina sequencing. Genomic DNA fragments produced by shearing by sonication are ligated to adaptors and amplified by polymerase chain reaction (PCR). The amplified DNA, separated by size and gel-purified, is suitable for use as template in whole-genome sequencing.

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