Indirect Immunometric ELISA | Zendy

Thomas O. Kohl | Zendy; Carl A. Ascoli | Zendy

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Indirect Immunometric ELISA

Author(s) -

Thomas O. Kohl,

Carl A. Ascoli

Publication year - 2017

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot093708

Subject(s) - chromogenic , analyte , chemiluminescence , antibody , antigen , reagent , substrate (aquarium) , chemistry , chromatography , fluorescence , microbiology and biotechnology , immunology , biology , ecology , physics , quantum mechanics

This assay facilitates the immunometric determination of assay-associated reagents including the capture and detection antibodies as well as the analyte (i.e., antigen or antibody). It can precede the development of a sandwich enzyme-linked immunosorbent assay (ELISA) in which optimal antibody concentrations are applied for the quantitative measurement of the antigen. This protocol describes the materials and equipment required for the measurement of chromogenic substrate development; however, it can be adapted for use with chemiluminescent- and fluorescent-labeled reporters.

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