Open AccessIsolation of High-Molecular-Weight DNA Using Organic Solvents
Author(s) -
Michael R. Green,
Joseph Sambrook
Publication year - 2017
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.prot093450
Subject(s) - nucleic acid , chloroform , nucleic acid methods , dna , chemistry , isolation (microbiology) , phenol , cloning (programming) , chromatography , biochemistry , combinatorial chemistry , organic chemistry , biology , bioinformatics , computer science , programming language
Perhaps the most basic of all procedures in molecular cloning is the purification of nucleic acids. The key step, the removal of proteins, can often be performed simply by extracting aqueous solutions of nucleic acids with phenol:chloroform and chloroform.
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