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Observation of Fluorescent Proteins in Fixed Cells
Author(s) -
Richard R. Behringer,
Marina Gertsenstein,
Kristina Vintersten Nagy,
András Nagy
Publication year - 2019
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.prot092775
Subject(s) - green fluorescent protein , fluorescence , fusion protein , fluorescent protein , fusion gene , microbiology and biotechnology , gene , chemistry , biology , computational biology , biochemistry , physics , recombinant dna , quantum mechanics
Fluorescent proteins (FPs) are popular reporters available for gene expression detection and determination of cellular identities in the mouse. This protocol can be used to detect green fluorescent protein spectral variants and proteins labeled with the fusion tag dsRed in fixed cells.

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