Open AccessObservation of Fluorescent Proteins in Living Cells
Author(s) -
Richard R. Behringer,
Marina Gertsenstein,
Kristina Vintersten Nagy,
András Nagy
Publication year - 2019
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.prot092767
Subject(s) - fluorescence , green fluorescent protein , embryonic stem cell , fluorescence microscope , fluorescent protein , microbiology and biotechnology , chemistry , cell , cell culture , biophysics , biology , biochemistry , gene , genetics , physics , quantum mechanics
Frequently, there is a need for fluorescent protein detection in mouse cell cultures, including embryonic stem cells or their differentiated derivatives, primary and transformed cells. Here, cells expressing green fluorescent protein-labeled proteins are observed using fluorescent microscopy.
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