Reprogramming Mouse Fibroblasts with piggyBac Transposons | Zendy

Richard R. Behringer | Zendy; Marina Gertsenstein | Zendy; Kristina Vintersten Nagy | Zendy; András Nagy | Zendy

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Reprogramming Mouse Fibroblasts with piggyBac Transposons

Author(s) -

Richard R. Behringer,

Marina Gertsenstein,

Kristina Vintersten Nagy,

András Nagy

Publication year - 2017

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot092627

Subject(s) - reprogramming , induced pluripotent stem cell , embryonic stem cell , microbiology and biotechnology , somatic cell , biology , transfection , stem cell , gene , genetics

In 2006, Shinya Yamanaka and his student Kazutoshi Takahashi showed that the expression of only four specific genes is sufficient to reprogram fully differentiated somatic cells into pluripotent stem cells. These cells, termed induced pluripotent stem (iPS) cells, share many of their characteristics with embryonic stem (ES) cells. In this protocol, we describe one of the simplest ways of generating iPS cells from mouse fibroblasts. It combines an efficient transposon-mediated transfection and the tetracycline-inducible system to control the expression of the Yamanaka reprogramming factors.

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