DNA Preparation from Schizosaccharomyces pombe | Zendy

Assen Roguev | Zendy; Jiewei Xu | Zendy; Nevan J. Krogan | Zendy

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DNA Preparation from Schizosaccharomyces pombe

Author(s) -

Assen Roguev,

Jiewei Xu,

Nevan J. Krogan

Publication year - 2017

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot091959

Subject(s) - schizosaccharomyces pombe , genotyping , polymerase chain reaction , genomic dna , dna , multiple displacement amplification , computational biology , schizosaccharomyces , dna extraction , biology , microbiology and biotechnology , chemistry , genetics , yeast , gene , genotype , saccharomyces cerevisiae

This protocol includes two methods for genomic DNA preparation from Schizosaccharomyces pombe . The first is a quick method for preparation of DNA suitable for polymerase chain reaction (PCR) genotyping. The second, longer method yields high-quality DNA that can be used for amplification of targeting cassettes.

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