Transformation of Schizosaccharomyces japonicus | Zendy

Keita Aoki | Zendy; Hironori Niki | Zendy

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Transformation of Schizosaccharomyces japonicus

Author(s) -

Keita Aoki,

Hironori Niki

Publication year - 2017

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot091850

Subject(s) - electroporation , plasmid , transformation (genetics) , schizosaccharomyces pombe , gene , homologous recombination , complementation , genetics , dna , biology , microbiology and biotechnology , mutant

This protocol describes the use of electroporation to transform Schizosaccharomyces japonicus with plasmids or linear DNA. Plasmids are helpful for the complementation testing of mutations and for the expression of specific genes. Linear DNA fragments integrated into chromosomal DNA by homologous recombination are useful for gene deletion or to fuse a gene with a tag sequence (e.g., encoding a fluorescent protein). To introduce DNA into S. japonicus , electroporation methods are recommended because S. japonicus is sensitive to lithium acetate (LiOAc).

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