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We present a protocol for metabolomic sample measurement using hydrophilic interaction chromatography (HILIC) combined with high-resolution Orbitrap mass spectrometry (MS). We also introduce a raw data processing method using MZmine 2 software, and include a list of 111 metabolite peaks (with their  m / z  values and retention times) previously identified in metabolome samples using this method.

Measurement of Metabolome Samples Using Liquid Chromatography–Mass Spectrometry, Data Acquisition, and Processing