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Measurement of Metabolome Samples Using Liquid Chromatography–Mass Spectrometry, Data Acquisition, and Processing
Author(s) -
Tomáš Pluskal,
Mitsuhiro Yanagida
Publication year - 2016
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.prot091561
Subject(s) - metabolome , orbitrap , chromatography , mass spectrometry , metabolomics , hydrophilic interaction chromatography , metabolite , chemistry , liquid chromatography–mass spectrometry , resolution (logic) , high performance liquid chromatography , computer science , artificial intelligence , biochemistry
We present a protocol for metabolomic sample measurement using hydrophilic interaction chromatography (HILIC) combined with high-resolution Orbitrap mass spectrometry (MS). We also introduce a raw data processing method using MZmine 2 software, and include a list of 111 metabolite peaks (with their m / z values and retention times) previously identified in metabolome samples using this method.

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