Tips on Hybridizing, Washing, and Scanning Affymetrix Microarrays | Zendy

Manuel Ares | Zendy

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Tips on Hybridizing, Washing, and Scanning Affymetrix Microarrays

Author(s) -

Manuel Ares

Publication year - 2014

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot080499

Subject(s) - scanner , standardization , computer science , microfluidics , automation , dna microarray , embedded system , fluidics , protocol (science) , barcode , computer hardware , nanotechnology , materials science , biology , engineering , operating system , artificial intelligence , mechanical engineering , gene , genetics , gene expression , aerospace engineering , medicine , alternative medicine , pathology

Starting in the late 1990s, Affymetrix, Inc. produced a commercial system for hybridizing, washing, and scanning microarrays that was designed to be easy to operate and reproducible. The system used arrays packaged in a plastic cassette or chamber in which the prefabricated array was mounted and could be filled with fluid through resealable membrane ports either by hand or by an automated "fluidics station" specially designed to handle the arrays. A special rotating hybridization oven and a specially designed scanner were also required. Primarily because of automation and standardization the Affymetrix system was and still remains popular. Here, we provide a skeleton protocol with the potential pitfalls identified. It is designed to augment the protocols provided by Affymetrix.

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