Open AccessPreparation of Rough Microsomes from Rat Liver
Author(s) -
David D. Sabatini
Publication year - 2014
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.prot079970
Subject(s) - microsome , polysome , translation (biology) , ribonuclease , in vitro , biochemistry , chemistry , function (biology) , membrane , rna , biology , microbiology and biotechnology , ribosome , messenger rna , gene
This protocol describes how to prepare rat liver rough microsomes that contain undegraded membrane-bound polysomes and can function very well in an in vitro translation system. It uses endogenous ribonuclease inhibitor in all steps, avoiding pelleting rough microsomes in all steps and sacrificing good recovery.
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