Creation of Primary Cell Lines from Lineage-Labeled Mouse Models of Cancer | Zendy

Andrew D. Rhim | Zendy

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Creation of Primary Cell Lines from Lineage-Labeled Mouse Models of Cancer

Author(s) -

Andrew D. Rhim

Publication year - 2015

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot078287

Subject(s) - lineage (genetic) , pancreatic cancer , biology , ex vivo , cell culture , cell , microbiology and biotechnology , gene , cancer research , cancer , in vivo , computational biology , genetics

Frequently, it is necessary to isolate pure populations of cancer cells for downstream assays, such as transcriptional analysis, signaling studies, and the creation of noncontaminated primary cell lines. Genetic lineage labeling with fluorescent reporter alleles allows for the identification of epithelial-derived cells within tumors. This protocol describes a method to isolate lineage-labeled pancreatic epithelial cells for ex vivo analysis, but it can be adapted for any type of lineage-labeled tumor.

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