Measuring Interstitial Diffusion, Convection, and Binding Parameters in Mouse Tumors | Zendy

Rakesh K. Jain | Zendy; Lance L. Munn | Zendy; Dai Fukumura | Zendy

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Measuring Interstitial Diffusion, Convection, and Binding Parameters in Mouse Tumors

Author(s) -

Rakesh K. Jain,

Lance L. Munn,

Dai Fukumura

Publication year - 2013

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot075721

Subject(s) - fluorescence recovery after photobleaching , photobleaching , diffusion , microscopy , chemistry , biophysics , fluorescence microscope , fluorescence , pathology , materials science , nuclear magnetic resonance , biomedical engineering , optics , biology , medicine , physics , thermodynamics

Noninvasive techniques have been developed for the assessment of various parameters in normal and diseased tissues of mice. This protocol describes the measurement of extravascular parameters, including interstitial diffusion, convection, and binding parameters, in mouse tumors. A fluorescently labeled molecule of interest is infused into the tumor interstitium, followed by imaging using single-photon microscopy or multiphoton laser-scanning microscopy (MPLSM). Fluorescence recovery after photobleaching (FRAP) with spatial Fourier analysis is performed. To measure interstitial diffusion coefficients, multiphoton FRAP is performed.

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