Open AccessAnalysis of Pre-mRNA Splicing Using HeLa Cell Nuclear Extracts
Author(s) -
Timothy W. Nilsen
Publication year - 2013
Publication title -
cold spring harbor protocols
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 51
eISSN - 1940-3402
pISSN - 1559-6095
DOI - 10.1101/pdb.prot075184
Subject(s) - rna splicing , hela , rna , messenger rna , alternative splicing , cell nucleus , polyacrylamide gel electrophoresis , microbiology and biotechnology , precursor mrna , chemistry , cell , polyacrylamide , biology , biochemistry , gene , enzyme
This protocol is used to determine the splicing behavior of pre-mRNAs in cell extracts that are capable of carrying out splicing (e.g., nuclear extracts from HeLa cells). (32)P-labeled RNA is incubated under splicing conditions for various times, and the resulting products are analyzed on denaturing polyacrylamide gels.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom