Purification of Astrocytes from Transgenic Rodents by Fluorescence-Activated Cell Sorting | Zendy

Lynette C. Foo | Zendy

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Purification of Astrocytes from Transgenic Rodents by Fluorescence-Activated Cell Sorting

Author(s) -

Lynette C. Foo

Publication year - 2013

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot074229

Subject(s) - cell sorting , astrocyte , green fluorescent protein , transgene , sorting , genetically modified mouse , microbiology and biotechnology , gene expression , biology , fluorescence , cell , gene , chemistry , flow cytometry , genetics , neuroscience , central nervous system , physics , quantum mechanics , computer science , programming language

The purification of astrocytes by fluorescence-activated cell sorting (FACS) requires that an astrocyte-specific promoter drive the expression of the green fluorescent protein (GFP). Our laboratory uses FACS to acutely isolate astrocytes from young and old tissue as well as to isolate GFP-negative neurons at the end of the FACS sorting to conduct comparative unbiased, large-scale gene expression studies. Because of the relatively harsh nature of FACS sorting, few astrocytes or neurons survive long enough after the sort to be cultured.

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