Gel Purification of RNA | Zendy

Timothy W. Nilsen | Zendy

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Gel Purification of RNA

Author(s) -

Timothy W. Nilsen

Publication year - 2013

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.prot072942

Subject(s) - rna , in vitro , gel electrophoresis , nucleotide , chemistry , transcription (linguistics) , biology , microbiology and biotechnology , computational biology , biochemistry , gene , linguistics , philosophy

For many applications, including size selection of RNAs and purification of in vitro transcription products, it is necessary to purify RNAs on a denaturing gel. This procedure describes how to purify transcripts that have been synthesized in vitro. It is useful for labeled or unlabeled RNAs when sufficient mass is present. It can also be used to isolate small RNAs. In general, RNA purification by denaturing gel electrophoresis is practical only when the size of the desired RNA is 600 nucleotides or less.

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