Designing and Troubleshooting Immunopanning Protocols for Purifying Neural Cells | Zendy

Ben A. Barres | Zendy

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Designing and Troubleshooting Immunopanning Protocols for Purifying Neural Cells

Author(s) -

Ben A. Barres

Publication year - 2014

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.ip073999

Subject(s) - troubleshooting , neuroscience , computer science , neuron , central nervous system , biology , operating system

Purifying and culturing cells from the central nervous system (CNS) has proved to be an incredibly powerful tool for dissecting fundamental neuron and glial properties, and especially powerful in understanding neuronal–glial interactions. In a series of detailed protocols, we have provided step-by-step instructions for purifying and culturing specific types of neurons, glia, and vascular cells from the CNS by immunopanning. This article discusses common pitfalls and errors as well as important design considerations for the immunopanning procedure.

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