Tips for Preparing mRNA-Seq Libraries from Poly(A)+ mRNA for Illumina Transcriptome High-Throughput Sequencing | Zendy

Brenton R. Graveley | Zendy

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Tips for Preparing mRNA-Seq Libraries from Poly(A)⁺ mRNA for Illumina Transcriptome High-Throughput Sequencing

Author(s) -

Brenton R. Graveley

Publication year - 2013

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.ip073908

Subject(s) - troubleshooting , transcriptome , illumina dye sequencing , messenger rna , throughput , computational biology , dna sequencing , computer science , biology , gene , gene expression , genetics , operating system , wireless

Many investigators who do high-throughput sequencing of mRNA (mRNA-Seq) use kits for library preparation purchased from Illumina. Recognizing that these kits are continually being updated and improved, we provide here some background information, tips, and troubleshooting advice for the kits available at the time of this writing.

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