General Considerations for Live Imaging of Developing Hippocampal Neurons in Culture | Zendy

Stefanie Kaech | Zendy; Chun-Fang Huang | Zendy; Gary Banker | Zendy

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General Considerations for Live Imaging of Developing Hippocampal Neurons in Culture

Author(s) -

Stefanie Kaech,

Chun-Fang Huang,

Gary Banker

Publication year - 2012

Publication title -

cold spring harbor protocols

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.674

H-Index - 51

eISSN - 1940-3402

pISSN - 1559-6095

DOI - 10.1101/pdb.ip068221

Subject(s) - green fluorescent protein , hippocampal formation , transfection , homogeneous , neuroscience , synapse , hippocampus , microbiology and biotechnology , polarity (international relations) , cell culture , computer science , biology , cell , gene , physics , genetics , thermodynamics

Dissociated cell cultures of the rodent hippocampus have become a standard model for studying many facets of neural development, including the development of polarity, axonal and dendritic growth, and synapse formation. The cultures are quite homogeneous--∼90% of the cells are pyramidal neurons--and it is relatively easy to express green fluorescent protein (GFP)-tagged proteins by transfection. This article describes the cultures and the key features of the system used to image them. It also includes suggestions on labeling cells with GFP-tagged proteins. It concludes with a discussion of the advantages and disadvantages of this culture system.

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