Using an Aplysia Two-Hybrid System to Examine the Interactions Between Transcription Factors Involved in Long-Term Facilitation in the Nervous System of Aplysia | Zendy

JungHwan Choi | Zendy; Jina Lee | Zendy; Seok-Won Yim | Zendy; ChaeSeok Lim | Zendy; ChiHoon Lee | Zendy; YoungDon Lee | Zendy; Dušan Bartsch | Zendy; Eric R. Kandel | Zendy; BongKiun Kaang | Zendy

Open Access

Using an Aplysia Two-Hybrid System to Examine the Interactions Between Transcription Factors Involved in Long-Term Facilitation in the Nervous System of Aplysia

Author(s) -

JungHwan Choi,

Jina Lee,

Seok-Won Yim,

ChaeSeok Lim,

ChiHoon Lee,

YoungDon Lee,

Dušan Bartsch,

Eric R. Kandel,

BongKiun Kaang

Publication year - 2003

Publication title -

learning and memory

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.228

H-Index - 136

eISSN - 1549-5485

pISSN - 1072-0502

DOI - 10.1101/lm.55303

Subject(s) - aplysia , facilitation , neuroscience , nervous system , transcription (linguistics) , transcription factor , biology , chemistry , microbiology and biotechnology , genetics , gene , philosophy , linguistics

Interactions between ApCREB1a, ApCREB2, and ApC/EBP have been studied using conventional methods such as the yeast two-hybrid system. However, it is unclear whether these memory-related transcription factors actually interact in the native environment in neurons. To clarify this question, we have developed an Aplysia two-hybrid system and found, consistent with previous studies that ApCREB2 interacts with ApCREB1a and ApC/EBP, and that ApC/EBP forms homodimers. We have also found that ApCREB1a and ApC/EBP do not interact. Therefore, our study shows that formerly described interactions between the proteins actually occur in the Aplysia neurons and that interactions between these transcription factors are specific.

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