An Automated Sample Preparation System for Large-Scale DNA Sequencing | Zendy

Andre Marziali | Zendy; T. D. Willis | Zendy; Nancy A. Federspiel | Zendy; Ronald W. Davis | Zendy

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An Automated Sample Preparation System for Large-Scale DNA Sequencing

Author(s) -

Andre Marziali,

T. D. Willis,

Nancy A. Federspiel,

Ronald W. Davis

Publication year - 1999

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.9.5.457

Subject(s) - biology , dna sequencing , throughput , template , sample (material) , computational biology , dna , scale (ratio) , computer science , genetics , nanotechnology , operating system , chromatography , materials science , chemistry , physics , quantum mechanics , wireless

Recent advances in DNA sequencing technologies, both in the form of high lane-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and high throughput. As part of the development of a fully automated sequencing system, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA templates per day from libraries of M13 plaques at a cost of $0.29 per sample. This Front End has been in high throughput operation since June, 1997 and has produced > 400,000 high-quality DNA templates.

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