Sequencing of cDNA Clones from the Genetic Map of Tomato (Lycopersicon esculentum) | Zendy

Martin W. Ganal | Zendy; Rosemarie Czihal | Zendy; Ulrich Hannappel | Zendy; Dorothee-U. Kloos | Zendy; Andreas Polley | Zendy; HongQing Ling | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Sequencing of cDNA Clones from the Genetic Map of Tomato (Lycopersicon esculentum)

Author(s) -

Martin W. Ganal,

Rosemarie Czihal,

Ulrich Hannappel,

Dorothee-U. Kloos,

Andreas Polley,

HongQing Ling

Publication year - 1998

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.8.8.842

Subject(s) - biology , lycopersicon , genetics , complementary dna , gene , dna sequencing , accession number (library science) , expressed sequence tag , gene mapping , restriction fragment length polymorphism , genetic linkage map , genbank , polymerase chain reaction , botany , chromosome

The dense RFLP linkage map of tomato ( Lycopersicon esculentum ) contains >300 anonymous cDNA clones. Of those clones, 272 were partially or completely sequenced. The sequences were compared at the DNA and protein level to known genes in databases. For 57% of the clones, a significant match to previously described genes was found. The information will permit the conversion of those markers to STS markers and allow their use in PCR-based mapping experiments. Furthermore, it will facilitate the comparative mapping of genes across distantly related plant species by direct comparison of DNA sequences and map positions. [cDNA sequence data reported in this paper have been submitted to the EMBL database under accession nos. AA824695 – AA825005 and the dbEST_Id database under accession nos. 1546519–1546862.]

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research