Alternate Polyadenylation in Human mRNAs: A Large-Scale Analysis by EST Clustering | Zendy

Daniel Gautheret | Zendy; Olivier Poirot | Zendy; Fabrice Lopez | Zendy; Stéphane Audic | Zendy; JeanMichel Claverie | Zendy

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Alternate Polyadenylation in Human mRNAs: A Large-Scale Analysis by EST Clustering

Author(s) -

Daniel Gautheret,

Olivier Poirot,

Fabrice Lopez,

Stéphane Audic,

JeanMichel Claverie

Publication year - 1998

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.8.5.524

Subject(s) - polyadenylation , biology , messenger rna , genetics , complementary dna , expressed sequence tag , computational biology , gene , cleavage and polyadenylation specificity factor , cluster analysis , microbiology and biotechnology , machine learning , computer science

Alternate polyadenylation is an important post-transcriptional regulatory process now open to large-scale analysis by use of cDNA databases. We clustered 164,000 expressed sequence tags (ESTs) into ∼15,000 groups and aligned each group to a putative mRNA 3′ end. By use of stringent criteria to discard artifactual mRNA extremities, clear evidence for alternate polyadenylation was obtained in 189 of the 1000 EST clusters studied. A number of previously unreported polyadenylation sites were identified, together with possible instances of tissue-specific differential polyadenylation. This study demonstrates that, besides quantitative aspects of gene expression, the distribution of alternate mRNA forms can be analyzed through EST sampling.

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