Characterization of Short Tandem Repeats from Thirty-One Human Telomeres | Zendy

Marjorie Rosenberg | Zendy; Lester Hui | Zendy; Junli Ma | Zendy; H. C. Nusbaum | Zendy; Kevin Clark | Zendy; Louise Robinson | Zendy; Laura Dziadzio | Zendy; Pamela Swain | Zendy; Tim P. Keith | Zendy; Thomas J. Hudson | Zendy; Leslie G. Biesecker | Zendy; Jonathan Flint | Zendy

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Characterization of Short Tandem Repeats from Thirty-One Human Telomeres

Author(s) -

Marjorie Rosenberg,

Lester Hui,

Junli Ma,

H. C. Nusbaum,

Kevin Clark,

Louise Robinson,

Laura Dziadzio,

Pamela Swain,

Tim P. Keith,

Thomas J. Hudson,

Leslie G. Biesecker,

Jonathan Flint

Publication year - 1997

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.7.9.917

Subject(s) - biology , cosmid , telomere , microsatellite , genetics , tandem repeat , chromosome , sequence (biology) , genome , human genome , sequence tagged site , repeated sequence , gene mapping , computational biology , dna , gene , allele

Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome.

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